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topflash  (Upstate Biotechnology Inc)
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Upstate Biotechnology Inc topflash
FIG. 4. Accumulation of - and -catenin during dedifferentiation of articular chondrocytes. A–C, articular chondrocytes were treated with the indi- cated concentrations of Wnt-7a-condi- tioned medium, LiCl, or interleukin-1 (IL-1) for 48 h. Levels of -catenin and -catenin were determined by Western blotting (A). Transcriptional activity of -catenin was determined using active <t>(TOPFLASH)</t> or inactive <t>(FOPFLASH)</t> <t>Tcf/Lef</t> reporter genes (B). Type II colla- gen expression was determined by West- ern blotting (C). ERK was employed as a loading control. D, chondrocytes were treated with 200 l of Wnt 7a-conditioned medium, 30 mM LiCl, or 5 ng/ml interleu- kin-1 for 24 h followed by determination of transcript levels of - and -catenin by RT-PCR (upper panel) and proteasome ac- tivity (lower panel). GAPDH, glyceralde- hyde 3-phosphate dehydrogenase.
Topflash, supplied by Upstate Biotechnology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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FIG. 4. Accumulation of - and -catenin during dedifferentiation of articular chondrocytes. A–C, articular chondrocytes were treated with the indi- cated concentrations of Wnt-7a-condi- tioned medium, LiCl, or interleukin-1 (IL-1) for 48 h. Levels of -catenin and -catenin were determined by Western blotting (A). Transcriptional activity of -catenin was determined using active (TOPFLASH) or inactive (FOPFLASH) Tcf/Lef reporter genes (B). Type II colla- gen expression was determined by West- ern blotting (C). ERK was employed as a loading control. D, chondrocytes were treated with 200 l of Wnt 7a-conditioned medium, 30 mM LiCl, or 5 ng/ml interleu- kin-1 for 24 h followed by determination of transcript levels of - and -catenin by RT-PCR (upper panel) and proteasome ac- tivity (lower panel). GAPDH, glyceralde- hyde 3-phosphate dehydrogenase.

Journal: Journal of Biological Chemistry

Article Title: Regulation of β-Catenin Signaling and Maintenance of Chondrocyte Differentiation by Ubiquitin-independent Proteasomal Degradation of α-Catenin

doi: 10.1074/jbc.m413367200

Figure Lengend Snippet: FIG. 4. Accumulation of - and -catenin during dedifferentiation of articular chondrocytes. A–C, articular chondrocytes were treated with the indi- cated concentrations of Wnt-7a-condi- tioned medium, LiCl, or interleukin-1 (IL-1) for 48 h. Levels of -catenin and -catenin were determined by Western blotting (A). Transcriptional activity of -catenin was determined using active (TOPFLASH) or inactive (FOPFLASH) Tcf/Lef reporter genes (B). Type II colla- gen expression was determined by West- ern blotting (C). ERK was employed as a loading control. D, chondrocytes were treated with 200 l of Wnt 7a-conditioned medium, 30 mM LiCl, or 5 ng/ml interleu- kin-1 for 24 h followed by determination of transcript levels of - and -catenin by RT-PCR (upper panel) and proteasome ac- tivity (lower panel). GAPDH, glyceralde- hyde 3-phosphate dehydrogenase.

Article Snippet: Briefly, cells were transiently trans- fected with 1 g of the Tcf/Lef reporter genes, TOPFlash (optimal Lef-binding site) or FOPFlash (mutated Lef-binding site) (Upstate Biotechnology Inc., Lake Placid, NY), and l g of pCMV- -galactosidase.

Techniques: Western Blot, Activity Assay, Expressing, Control, Reverse Transcription Polymerase Chain Reaction